One-step coating of silica capillaries for selective protein retention by Cu(II)-IDA IMAC

► Synthesis and full characterization of IDA-silane. ► Elaboration of IMAC columns by a one step coating of the synthesized silane. ► First characterization of IDA content of the coated capillaries. ► Selectivity of our Cu(II)-supports towards holotransferrin was shown. ► Compatibility of IMAC with HPLC was shown.