A specific probe for two-photon fluorescence lysosomal imaging

► In this manuscript, we reported that a lysosomal probe derived from Tröger's base for two-photon fluorescence imaging was designed and synthesized. ► This probe showed high specificity and selectivity for lysosomes, and the Pearson's correlation coefficient is 0.96. ► It also provided high resolution 3D reconstruction of lysosomes in one HeLa cell by using two-photon confocal microscopy.