کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7689150 1495889 2016 45 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative metabolic network profiling of Escherichia coli: An overview of analytical methods for measurement of intracellular metabolites
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitative metabolic network profiling of Escherichia coli: An overview of analytical methods for measurement of intracellular metabolites
چکیده انگلیسی
Understanding the mechanism of metabolic network response to extracellular changes has become one of the primary objectives of systems biology. Quantitative metabolic network profiling of model organisms such as E. coli is the principal approach. Well-designed analytical protocols for the determination of intracellular metabolites may provide a crucial premise to quantitative profiling of cellular metabolism. This article reviews and evaluates the existing methods for determining intracellular metabolites of E. coli in terms of sample preparation and mass spectrometry (MS) analysis. Critical issues including the variation of metabolic status and interference of external pools during cell sampling, sample loss during quenching procedures, degradation of metabolites and efficiency of extraction, and matrix effects in separation and quantification are discussed. Moreover, suggestions are provided to solve the analytical problems based on the results from the recent studies in order to establish a precise and reliable analytical platform for the intracellular metabolites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 75, January 2016, Pages 141-150
نویسندگان
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