Theoretical and practical interest in UHPLC technology for 2D-LC

Comprehensive two-dimensional liquid chromatography (LC x LC) has significantly improved the separation power of LC for complex samples. Our review opens with theoretical considerations about on-line LC x LC in order to explain why ultra-high-pressure LC (UHPLC) and more specifically, high-temperature (HT)-UHPLC are very convenient in the second dimension. We present some recent applications involving UHPLC in LC x LC. They cover a wide range of analytical fields, such as food analysis, life sciences, bioenergy and polymers.