Probing microscopic material properties inside simulated membranes through spatially resolved three-dimensional local pressure fields and surface tensions

► This article presents methodology to calculate 3D-resolved pressure in simulations. ► We have implemented this methodology in the GROMACS software. ► Lipid membranes are a soft material with local fluctuations in pressure. ► Membrane proteins exert a detectable local perturbation on their lipid environment.