Humanlike substitutions to Ω-loop D of yeast iso-1-cytochrome c only modestly affect dynamics and peroxidase activity

Humanlike A81I and G83V substitutions to yeast iso-1-cytochrome c slow alkaline isomerization and decrease the peroxidase activity of cytochrome c at pH 7 and 8, which is important early in apoptosis. Acid-induced isomerization can also increase access to conformers competent for peroxidase activity.328