کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7781211 | 1500558 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Affecting parameters on fabrication of β-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Affecting parameters on fabrication of β-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers Affecting parameters on fabrication of β-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers](/preview/png/7781211.png)
چکیده انگلیسی
β-galactosidase was successfully immobilized onto chitosan (CS)/polyvinyl alcohol (PVA) blend nanofibers through electrospinning. The effects of both polymer solution parameters and the electrospinning process conditions on the morphology of nanofibers and enzyme activity were studied. FESEM results showed that both morphology and diameter of the nanofibers were significantly influenced by the studied factors. The nanofiber mats obtained from CS/PVA at 50:50 mass ratio and conditions of 20âkV and 0.5âml/h were selected as the optimized nanofibers, with the smallest diameter and most homogenous structure. On the other hand, catalytic activity of the β-galactosidase -immobilized nanofibers (Enzyme-NF) was not significantly changed with these factors. The catalytic activity of Enzyme-NF was 57.03% of free enzyme activity at pH 6.8 and 4â°C. Furthermore, it showed more temperature stability compared to free β-galactosidase at 50â°C. During 28 days of storage, Enzyme-NF samples retained 77% and 42% of its initial activity in 4â°C and 25â°C, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 200, 15 November 2018, Pages 137-143
Journal: Carbohydrate Polymers - Volume 200, 15 November 2018, Pages 137-143
نویسندگان
Sara Haghju, Mahmoud Rezazadeh Bari, Mohammad Alizadeh Khaled-Abad,