کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7787327 | 1500624 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 â 3)-linked β-d-glucan oligosaccharides
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to find an efficient enzymatic tool for curdlan degradation to produce (1 â 3)-linked β-d-glucan oligosaccharides, strain E4-5 (registration number JN089883, Genbank) was isolated from seaside soil. The 16S rRNA gene sequencing classified it as Cellulosimicrobium cellulans. It was the first reported microorganism that succeeded in degrading high-set heated curdlan blocks. The ferments of strain E4-5 also showed good degradation effects on laminaran and alkali-neutralized curdlan. Due to the products with less amount of glucose, it was assumed that endo-1,3-β-glucanases of strain E4-5 had a greater hydrolyzing effect than exo-1,3-β-glucanases. This indicated that strain E4-5 was a promising microorganism to hydrolyze (1 â 3)-linked β-d-glucan. Moreover, alkali-neutralization pretreatment was effective for promoting a more diversified degree of polymerization (DP) of (1 â 3)-linked β-d-glucan oligosaccharides under enzymatic hydrolysis and will pave the way for making full use of curdlan for production of glucan oligosaccharides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 134, 10 December 2015, Pages 740-744
Journal: Carbohydrate Polymers - Volume 134, 10 December 2015, Pages 740-744
نویسندگان
Yunbin Fu, Likun Cheng, Yanyu Meng, Shuguang Li, Xiaoming Zhao, Yuguang Du, Heng Yin,