کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7790262 | 1500643 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Wood mimetic hydrogel beads for enzyme immobilization
ترجمه فارسی عنوان
دانه های هیدروژل متی متیل برای غیر فعال شدن آنزیم
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کلمات کلیدی
چوب، کامپوزیت، هیدروژل، مایع یونی، لیپاز، ایمن سازی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility. The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization. Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution. Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood. The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability. The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively. Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 115, 22 January 2015, Pages 223-229
Journal: Carbohydrate Polymers - Volume 115, 22 January 2015, Pages 223-229
نویسندگان
Saerom Park, Sung Hee Kim, Keehoon Won, Joon Weon Choi, Yong Hwan Kim, Hyung Joo Kim, Yung-Hun Yang, Sang Hyun Lee,