کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7791145 | 1500645 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum
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کلمات کلیدی
NF-κBJnkp38COX-2ERKiNOSc-Jun N-terminal kinase - C-Jun N-terminal kinaseMAPK - MAPKMAPKs - MAPK هاinducible NO synthase - NO سنتاز القاء شدهGlycosidic linkage - ارتباط گلیکوزیدیinterleukin - اینترلوکینImmunomodulation - تنظیم ایمنی tumor necrosis factor-α - تومور نکروز عامل αCyclooxygenase-2 - سیکلوکوکسیژناز2TNF-α - فاکتور نکروز توموری آلفاNuclear factor-kappa B - فاکتور هسته ای-کاپا BNitric oxide - نیتریک اکسیدmitogen-activated protein kinases - کیناز پروتئین فعال Mitogen
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The sulfated-fucans, known as fucoidans, were isolated from Agarum cribrosum and fractionated using ion-exchange chromatography to determine their molecular characteristics and in vitro immunomodulatory activity. The crude and fractionated fucoidans (F1 and F2) consisted mostly of carbohydrates (52.4-56.0%), sulfates (12.7-23.0%) and uronic acid (14.1-21.8%), with a small amount of proteins (3.9-9.3%), and included various levels of fucose (44.0-46.7%), mannose (18.9-26.8%), galactose (16.8-33.0%), xylose (10.7-17.0%) and glucose (3.5-9.5%). The crude and fractionated fucans contained one or two subfractions with average molecular weights (Mw) ranging from 110.1 Ã 103 to 2420 Ã 103 g/mol. The fractionated fucoidan, especially the F1 fraction, strongly stimulated murine macrophages (Raw 264.7 cells), producing a considerable amount of nitric oxide (NO) and inducing expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and interleukin-10 (IL-10) transcripts by activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) pathways. The maximally immunoenhancing F1 fraction was mainly composed of (1 â 3)-linked fucose, (1 â 2)-linked mannose and (1 â 4)-linked glucuronic acid with sulfates at C-2 or both the C-2 and C-4 positions in (1 â 2,3)- and (1 â 2,3,4)-linked fucose residues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 113, 26 November 2014, Pages 507-514
Journal: Carbohydrate Polymers - Volume 113, 26 November 2014, Pages 507-514
نویسندگان
MyoungLae Cho, Dong-Jin Lee, Jin-Kyung Kim, SangGuan You,