Susceptibility of enoxaparin reducing end amino sugars to periodate oxidation

- 1,6-Anhydro-bearing tetrasaccharides were isolated from enoxaparin sample.
- Their susceptibility to periodate oxidation was evaluated by NMR and LC/MS.
- 1,6-Anhydro-bridge is crucial for oxidation of 1,6-anhydro-N-sulfo-mannosamine.
- Reducing end N-sulfate hexosamine is affected by NaIO4 under drastic conditions.
- It is possible to selectively split non-sulfated iduronic acids of enoxaparin chains.