Design and synthesis of NBD-S-dye dyads for fluorescently discriminative detection of biothiols and Cys/Hcy

Thiolysis of the NBD thioether was employed to develop highly sensitive and selective fluorescent probes for discriminative detection of biothiols and Cys/Hcy. The probe could eliminate the effect of probe consuption by GSH when used to detect Cys in PBS buffer and in living cells.127