Protein Biosensors Based on Scanning Potential Hairpin Denaturation and Fluorescence Resonance Energy Transfer

The ability of detecting multiple analyses simultaneously is an important advantage of protein sensors. But the denaturation of proteins on surfaces is still a challenge. In this article, a protein biosensor providing high selectivity is introduced. This approach is based on DNA-directed immobilization(DDI) method, in which protein-DNA conjugates are immobilized on surfaces by DNA hybridization. Nonspecific binding is reduced by applying fluorescence resonance energy transfer and scanning potential hairpin denaturizing technologies. Excellent discrimination on different recognition patterns is obtained.