کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7842907 1506516 2018 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spectroscopic studies on in vitro molecular interaction of highly fluorescent carbon dots with different serum albumins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Spectroscopic studies on in vitro molecular interaction of highly fluorescent carbon dots with different serum albumins
چکیده انگلیسی
Fluorescent Carbon Dots (CDs) have gained paramount interest in chemical biological and biomedical fields. In this work, in vitro molecular interaction of CDs with various serum albumins (Human serum albumins (HSA), Bovine serum albumins (BSA) and Rabbit serum albumins (RSA)) have been systematically studied by various spectroscopic techniques. Spherical and monodispersed CDs with an average diameter across of 10 nm successfully fabricated in water-(Polyethylene glycol) PEG medium utilizing glucose as carbon source. Thermodynamic and kinetic parameters of CDs with serum albumins (SA) have also been studied. The binding interaction between serum albumins (SA) and CDs were come about because of the complex formation of HSA-CDs, BSA-CDs and RSA-CDs. The altered Stern-Volmer quenching constant Ka at different temperatures and their comparing thermodynamic parameters ∆H, ∆G and ∆S were calculated. The binding location of CDs with SA is around site I (subdomain IIA) and substantial conformational changes at both secondary and tertiary structures level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Liquids - Volume 255, 1 April 2018, Pages 279-287
نویسندگان
, , , , ,