کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8087858 | 1521820 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A multifunctional lipoxygenase from Pyropia haitanensis- The cloned and functioned complex eukaryotic algae oxylipin pathway enzyme
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کلمات کلیدی
ALASPMEHpODEOxylipinHEPEPyropia haitanensislinoleicEPAHPLEST - استArachidonic acid - اسید آراشیدونیکEicosapentaenoic acid - اسید ایکوزاپنتانوئیکdocosahexaenoic acid - اسید داکوزاگزوائونیکLinolenic acid - اسید لینولینیکPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعLOX - اکسیژن مایعAOS - بهExpressed Sequence Tag - تکرار اظهار نظرARA - در حال حاضرDHA - دوکوساهگزائنوئیک اسیدAllene oxide synthase - سنتاز اکسید آلنlipoxygenase - لیپواکسیژنازlipoxygenases - لیپوکسیژنازهاsolid phase micro-extraction - میکرو استخراج فاز جامدHPETE - هتلیHETE - هفتهHydroperoxide lyase - هیدروپراکسید لیازMultifunction - چند منظوره
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A multifunctional lipoxygenase from Pyropia haitanensis- The cloned and functioned complex eukaryotic algae oxylipin pathway enzyme A multifunctional lipoxygenase from Pyropia haitanensis- The cloned and functioned complex eukaryotic algae oxylipin pathway enzyme](/preview/png/8087858.png)
چکیده انگلیسی
Lipoxygenases (LOXs) are critical starting biocatalysts for the synthesis of signaling compounds derived from lipid peroxidation. However, LOXs from prokaryotes or lower eukaryotes always show nonspecific and multifunctional properties. Here, an oxylipin pathway enzyme gene of eukaryotic algae, Pyropia haitanensis of Bangiaceae (Rhodophyta), named PhLOX was cloned and characterized; a phylogenetic analysis was also performed. This unique LOX isoform is a multifunctional enzyme, combined unusually high hydroperoxidelyase (HPL), lipoxygenase, and allene oxide synthase (AOS) three catalytic activities within one catalytic domain of the protein, which may explain the observed diversity of P. haitanensis oxylipins. Phylogenetic analysis indicated that red algae LOXs separated from the LOX clades of the ancestor of higher plant and animal in the early stages of evolution. The substrate flexibility, oxygenation position flexibility, and functional versatility of PhLOX represented typical properties of lower organisms. These results indicated that the origin of the oxylipin biosynthetic gene and the oxylipin biosynthetic pathway of some red algae are unique, which may shed light on the specific defense strategies of these red algae and the evolution of the oxylipin pathway as well as the compact genome of red algae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 12, November 2015, Pages 316-327
Journal: Algal Research - Volume 12, November 2015, Pages 316-327
نویسندگان
Hai-min Chen, Zhu-jun Zhu, Juan-Juan Chen, Rui Yang, Qi-jun Luo, Ji-lin Xu, He Shan, Xiao-Jun Yan,