GLUT12 functions as a basal and insulin-independent glucose transporter in the heart

► Use of a biotinylated photolabeled assay in perfused myocardium and cardiac myocytes ► Insulin increased translocation of GLUT-4, but not -12, in the healthy myocardium ► Diabetes induced alteration in insulin-stimulated GLUT-4, but not -12, translocation. ► Active cell surface GLUT12 was increased in the diabetic myocardium.