کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8267270 | 1534944 | 2016 | 51 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of Neurospora Catalase-3 by global heterochromatin formation and its proximal heterochromatin region
ترجمه فارسی عنوان
مقررات نوروسپورا کاتالاز-3 توسط تشکیل جهانی هتروکروماتین و منطقه پروتزیمال هتروکروماتین
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کلمات کلیدی
CATGSTChIP-qPCRHpo - HPONOx - NOXO2•− - O2 • -ROS - ROSHydrogen peroxide - آب اکسیژنهpost-translational modification - اصلاح post-translationalNADPH oxidase - اکسیداز NADPH Superoxide - سوپر اکسیدHeterochromatin - هتروکروماتینH2O2 - هیدروژن پراکسیدCatalase - کاتالازglutathione S-transferase - گلوتاتیون S-ترانسفرازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
Catalase-3 (CAT-3) constitutes the main catalase activity in growing hyphae of Neurospora crassa, and its activity increases during exponential growth or is induced under different stress conditions. Although extensive progress has been made to identify catalase regulators, the regulation mechanism of CAT-3 at the chromatin level still remains unclear. Here, we aim at investigating the molecular regulation mechanisms of cat-3 at the chromatin level. We found that CAT-3 protein levels increased in mutants defective in proper global heterochromatin formation. Bioinformatics analysis identified a 5-kb AT-rich sequence adjacent to the cat-3 promoter as a heterochromatin region because of its enrichment of H3K9me3 and HP1. Expression of CAT-3 was induced by H2O2 treatment in wild-type and such change occurred along with the accumulation of histone H3 acetylation at 5-kb heterochromatin boundaries and cat-3 locus, but without alteration of its H3K9me3 repressive modification. Moreover, disruption of 5-kb heterochromatin region results in elevated cat-3 expression, and higher levels of cat-3 expression were promoted by the combination with global heterochromatin defective mutants. Interestingly, the molecular weight and activity bands of CAT-3 protein are different in heterochromatin defective mutants compared with those in wild-type, suggesting that its N-terminal processing and modification may be altered. Our study indicates that the local chromatin structure creates a heterochromatin repressive environment to repress nearby gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 99, October 2016, Pages 139-152
Journal: Free Radical Biology and Medicine - Volume 99, October 2016, Pages 139-152
نویسندگان
Yajun Wang, Qing Dong, Zhaolan Ding, Kexin Gai, Xiaoyun Han, Farah Naz Kaleri, Qun He, Ying Wang,