Further evidence of the trypanocidal action of eupomatenoid-5: Confirmation of involvement of reactive oxygen species and mitochondria owing to a reduction in trypanothione reductase activity

► Eupomatenoid-5 has trypanocidal activity in three parasitic forms of T. cruzi. ► Eupomatenoid-5 induces reduction of trypanothione reductase activity in T. cruzi. ► Eupomatenoid-5 induces increase in the formation of oxidant species in T. cruzi. ► Eupomatenoid-5 induces mitochondrial depolarization in T. cruzi. ► Eupomatenoid-5 induces oxidative imbalance in T. cruzi followed by parasite death.