کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8284696 | 1535581 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of polyphenols from coffee and grape on gene expression in myoblasts
ترجمه فارسی عنوان
اثر پلی فنل از قهوه و انگور بر بیان ژن در میوبلاست ها
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کلمات کلیدی
PBSAHRDMEMFBSNrf2TPCGSHDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoROS - ROSAntioxidant - آنتی اکسیدانGrape - انگورDifferentiation - تفکیکfetal bovine serum - سرم جنین گاوphosphate buffer saline - فسفات بافر شورCoffee - قهوهTotal polyphenolic content - محتوای کل پلی فنلPolyphenols - پلیفنلهاGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژنaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
Coffee and grape contain various bioactive compounds like polyphenols that may exert beneficial effects, especially antioxidant activity, on human health upon consumption. However, the molecular mechanisms through which these effects are achieved are not fully elucidated. Thus, in the present study in order to investigate these mechanisms, a whole genome expression DNA microarray analysis was carried out in myoblasts treated with polyphenols of coffee and grape pomace at concentrations that improved the redox status. Grape was composed of catechin, epicatechin, cyanidin, malvidin, delphinidin, petunidin, myrtillin, kuromanin, oenin, peonidin, quercetin, gallic acid and caftaric acid as LC-MS revealed, with a total polyphenolic content (TPC) of 648â¯mg of gallic acid equivalents/g of dry matter. Coffee had a TPC of 42.61â¯mg GAE/g coffee and was composed of 3-chlorogenic acid (16.61â¯mg/g), 4- and 5-chlorogenic acids (13.62â¯mg/g), as UHPLC-HRMS revealed. According to the results, grape polyphenols altered mainly the expression of cytoskeleton and differentiation-associated genes, while coffee compounds had a more profound effect, on the expression levels of many metabolic and antioxidant genes possibly through the nuclear factor (erythroid-derived 2) like-2 (Nrf2) pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 172, June 2018, Pages 115-122
Journal: Mechanisms of Ageing and Development - Volume 172, June 2018, Pages 115-122
نویسندگان
Alexandros Priftis, Nikolaos Goutzourelas, Maria Halabalaki, Georgia Ntasi, Dimitrios Stagos, Grigorios D. Amoutzias, Leandros A. Skaltsounis, Dimitrios Kouretas,