کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8288930 | 1536270 | 2017 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prothymosin α interacts with SET, ANP32A and ANP32B and other cytoplasmic and mitochondrial proteins in proliferating cells
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کلمات کلیدی
STSN-terminalTα1ProTαMitochondrial apoptosis - آپوپتوز میتوکندریاییstaurosporine - استوسوسورپینC-terminal - ترمینال CCell proliferation - تکثیر سلولیThymosin α1 - تیموشین α1Flow-through - جریان از طریقProtein complex - پروتئین پیچیدهProthymosin alpha - پروتیموسین آلفاprothymosin α - پروتیوموزین αCross-linking - پیوند متقابل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Prothymosin α (ProTα) is an acidic protein with a nuclear role related to the chromatin activity through its interaction with histones in mammalian cells. ProTα acts as an anti-apoptotic factor involved in the control of the apoptosome activity in the cytoplasm, however the mechanisms underlying this function are still known. ProTα shares similar biological functions with acidic nuclear-cytoplasmic shuttling proteins included in SET and ANP32 family members. Using affinity chromatography, co-immunoprecipitation and chemical cross-linking, we demonstrate that ProTα interacts with SET, ANP32A and ANP32B proteins. The study by mass spectrometry of the complexes stabilized by chemical cross-linking showed that associations of ProTα consist of six highly acidic ProTα-complexes, which corresponds to differentiated interactions of ProTα either with SET or ANP32 proteins. The presence in the ProTα-complexes of cytoplasmic proteins involved in membrane remodeling and proteins implicated in the mitochondrial permeability, seems to indicate that they could be related to a cytoplasmic-mitochondrial activity. According to the cellular function of the characterized targets of ProTα, and the evolution in the composition of the diverse ProTα-complexes when proliferation activity was reduced or apoptosis induced, leads to hypothesized that ProTα interactions might be related to the proliferation activity and control of the cell survival.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 635, 1 December 2017, Pages 74-86
Journal: Archives of Biochemistry and Biophysics - Volume 635, 1 December 2017, Pages 74-86
نویسندگان
Pablo Barbeito, Concepción S. Sarandeses, Cristina DÃaz-Jullien, Juan Muras, Guillermo Covelo, David Moreira, Carmen Freire-Cobo, Manuel Freire,