Purification and characterization of a trehalase-invertase enzyme with dual activity from Candida utilis

► Dual active trehalase-invertase enzyme purified from Candida utilis. ► The native enzyme is a 175 kDa trimer. ► Chemically induced monomeric forms are inactive. ► Enzyme can hydrolyze sucrose more efficiently. ► The enzyme shows greater similarity to previously known neutral trehalases.