Molecular, kinetic, thermodynamic, and structural analyses of Mycobacterium tuberculosishisD-encoded metal-dependent dimeric histidinol dehydrogenase (EC 1.1.1.23)

► Mycobacterium tuberculosishisD-encoded histidinol dehydrogenase (MtHisD) is a dimeric metalloenzyme. ► MtHisD follows a Bi Uni Uni Bi Ping-Pong mechanism. ► l-Histidinol binds to free MtHisD. ► The imidazole group of His336 plays a critical role in catalysis and l-histidinol binding.