کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8293848 | 1536749 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell model for the identification and characterization of prion-like components from Alzheimer brain tissue
ترجمه فارسی عنوان
مدل سلول برای شناسایی و مشخص کردن اجزای پیک مانند از بافت مغز آلزایمر
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کلمات کلیدی
عصب مغزی پروتئین تاشو، غلظت پروتئین،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Intracerebral injection of brain extracts from Alzheimer's disease (AD) patients into appropriate mouse models was previously found to drastically accelerate the deposition of Aβ amyloid in the recipient animals indicating a prion-like activity. In this study we show that this prion-like activity can be also identified by using a cell culture model of Aβ plaque formation. Analysis of biochemical fractions of AD brain extract indicate that the seeding-activity correlated with the presence of Aβ peptide and Aβ-derived aggregates. In vitro-formed fibrils were also active but their activity was low and depending on the fibril structure and conditions of fibril formation. Our data indicate a conformational basis of the observed seeding effect and suggest the utility of our cell model for further studies on the prion-like activity of AD extracts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 3, 11 March 2018, Pages 857-862
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 3, 11 March 2018, Pages 857-862
نویسندگان
Daniel Markx, Cornelia Loos, Stephanie Claus, Christian Haupt, Christian Mawrin, Marcus Fändrich,