کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8294041 | 1536750 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of PQ's cytotoxicity on secretory vesicles in astroglia: Expression alternation of secretogranin II and its potential interaction with intracellular factors
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کلمات کلیدی
GLT-1TNFSecretogranin IIISecretogranin IIS100 calcium binding protein BAstrogliaGDNFIL-1βGFAPIL-6BDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز S100β - S100binterleukin 1 beta - اینترلوکین 1 بتاinterleukin 6 - اینترلوکین 6brain derived neurotrophic factor - عامل مغز استخوان مغز استخوان استGlial cell derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از سلول گلیالtumor necrosis factor - فاکتور نکروز تومورParaquat - پاراکواتGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالglutamate transporter-1 - گلوتامات transporter-1Glutamine synthetase - گلوتامین سنتتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
It has been extensively characterized that paraquat (PQ) selectively targets to the substantia nigra and exerts neurotoxic actions on dopaminergic neurons. However, a little knowledge is available about astroglia in PQ exposure, especially its complex secretory machinery. To explore this point, we built up a PQ-induced model in cultural U118 astrocyte. Since the granin family is considered as a master regulator of cargo sorting and large dense core vesicles (LDCVs) biogenesis in the regulated secretory pathway of nervous and neuroendocrine cells, the current study focused on one member, secretogranin II (SCG2) and investigated its alternation and potential relationship with other astrocyte-derived factors under PQ insult. We found that PQ upregulated SCG2 expression on both RNA and protein levels and stimulated the mRNA expression of neurotrophic factors, cytokines and glutamine synthetase (GS) simultaneously. RNAi knockdown of SCG2 did not rescue the cell cycle arrest induced by PQ but affected expressions of IL-6 and GS on mRNA and protein levels. Further studies on subcellular location showed that SCG2-positive secretory granules were partially colocalized with IL-6 but not GS in PQ exposure astrocyte. Taken together, our findings indicate that the expression alternation of SCG2 under astroglial activation by PQ may be necessary compensation for cargo sorting and LDCV biogenesis. The involvement of the IL-6 and GS suggests that the SCG2 may potentially regulate inflammatory factors and excitatory neurotransmitter to the cytotoxicity of PQ on astroglia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 2, 4 March 2018, Pages 675-682
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 2, 4 March 2018, Pages 675-682
نویسندگان
Xiaoni Zhan, Fengrui Li, Qiaohong Chu, Hao Pang,