کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8294475 | 1536753 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetic profiles of photocurrents in cells expressing two types of channelrhodopsin genes
ترجمه فارسی عنوان
پروفیل های جنبشی از جریان های فوتون در سلول ها بیان دو نوع ژن کانترودوپسین
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Channelrhodopsin-2 (ChR2), a light-activated cation-selective ion channel, has been widely used as a tool in optogenetic research. ChR2 is specifically sensitive to wavelengths less than 550â¯nm. One of the methods to expand the sensitivity of a channelrhodopsin to a wider range of wavelengths is to express another channelrhodopsin in the cells by the transduction of an additional gene. Here, we report the characteristic features of cells expressing two types of channelrhodopsins, each having different wavelength sensitivities. In HEK293â¯cells stably expressing ChR2, photocurrents were elicited at stimuli of 400-550â¯nm, and the wavelength sensitivity range was expanded by the additional transduction of the modified Volvox channelrhodopsin-1 (mVChR1) gene, which has broad wavelength sensitivities, ranging from 400 to 600â¯nm. However, the photocurrent at 550â¯nm was lower than that of the mVChR1-expressing cell; moreover, the turning-on and turning-off constants were delayed, and the deactivation rates were decreased. Meanwhile, the response to lower light intensity was improved by the additional gene. Thus, the transduction of an additional gene is a useful method to improve the light and wavelength sensitivities, as well as photocurrent kinetic profiles, of channelrhodopsins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 3, 12 February 2018, Pages 814-819
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 3, 12 February 2018, Pages 814-819
نویسندگان
Yoshito Watanabe, Eriko Sugano, Kitako Tabata, Taku Ozaki, Takehiko Saito, Makoto Tamai, Hiroshi Tomita,