کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8296347 1536764 2017 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biophysical characterization of the interaction between heme and proteins responsible for heme transfer in Streptococcus pyogenes
ترجمه فارسی عنوان
تعیین بیوفیزیکی تعامل بین هم و پروتئین های مسئول انتقال هموگلوبین در استرپتوکوک پیوژنز
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Streptococcus pyogenes, an important pathogen that causes a wide range of diseases, possesses the sia gene cluster, which encodes proteins involved in the heme acquisition system. Although this system was previously described, the molecular mechanism of effective heme transfer remains to be elucidated. Here, we have characterized the interactions between heme and each domain of Streptococcal hemoprotein receptor (Shr) and Streptococcal heme-binding protein (Shp). Our kinetic and thermodynamic analyses suggested that effective heme transfer within this system is achieved not only by affinity-based transfer but also by the difference of the binding driving force. The biophysical characterization of the above-mentioned interaction will lead to an indication for the selection of the target for a chemical screening of inhibitors as novel antibacterial agents based on biophysical approaches.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 493, Issue 2, 18 November 2017, Pages 1109-1114
نویسندگان
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