Src family kinase inhibitor PP2 accelerates differentiation in human intestinal epithelial cells

► A peak of Src activity is observed in early stages of Caco-2 cell differentiation. ► Inhibition of SFKs triggers the overall intestinal differentiation program. ► Up-regulation of both polarization and terminal differentiation markers is observed. ► A rise in Cdx2 and HNF1α levels and decrease of H3K27m3 are also noted. ► This suggests a key role for SFKs in intestinal cell terminal differentiation.