کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8298723 | 1537041 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress
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کلمات کلیدی
DAPIYPGal1-anilino-8-naphthalenesulfonic acidYPdNBD-PETMRMFCCPPMSFCFUHEPES4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولMitochondrial DNA - DNA میتوکندریاethidium bromide - اتیدیوم برومایدFluorescence resonance energy transfer - انتقال انرژی رزونانس FluorescenceFRET - انتقال انرژی رزونانسی فورسترmtDNA - دیانای میتوکندریاییphosphatidylcholine - فسفاتیدیل کولینphosphatidylglycerol - فسفاتیدیل گلیسرولphosphatidylethanolamine - فسفاتیدیلتانولامینPhosphatidylserine - فسفاتیدیلسرینPhospholipid - فسفولیپیدPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدAnsa - مورد علاقهMitochondrion - میتوکندریونwild type - نوع وحشیcolony forming units - واحدهای تشکیل دهنده کلنیCardiolipin - کاردیولیپین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress](/preview/png/8298723.png)
چکیده انگلیسی
Mitochondria play a key role in adaptation during stressing situations. Cardiolipin, the main anionic phospholipid in mitochondrial membranes, is expected to be a determinant in this adaptive mechanism since it modulates the activity of most membrane proteins. Here, we used Saccharomyces cerevisiae subjected to conditions that affect mitochondrial metabolism as a model to determine the possible role of cardiolipin in stress adaptation. Interestingly, we found that thermal stress promotes a 30% increase in the cardiolipin content and modifies the physical state of mitochondrial membranes. These changes have effects on mtDNA stability, adapting cells to thermal stress. Conversely, this effect is cardiolipin-dependent since a cardiolipin synthase-null mutant strain is unable to adapt to thermal stress as observed by a 60% increase of cells lacking mtDNA (Ï0). Interestingly, we found that the loss of cardiolipin specifically affects the segregation of mtDNA to daughter cells, leading to a respiratory deficient phenotype after replication. We also provide evidence that mtDNA physically interacts with cardiolipin both in S. cerevisiae and in mammalian mitochondria. Overall, our results demonstrate that the mitochondrial lipid cardiolipin is a key determinant in the maintenance of mtDNA stability and segregation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issues 6â7, JuneâJuly 2015, Pages 587-598
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issues 6â7, JuneâJuly 2015, Pages 587-598
نویسندگان
Luis Alberto Luévano-MartÃnez, Maria Fernanda Forni, Valquiria Tiago dos Santos, Nadja C. Souza-Pinto, Alicia J. Kowaltowski,