کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8299038 | 1537049 | 2010 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
LESOECChlRWCPQH2Haberlea rhodopensisFNRP700PheoFerredoxin-NADP+ reductase - Feredoxin-NADP + ردوکتازQA and QB - QA و QBJIP-test - آزمون JIPTyr - تیرChlorophyll - سبزینه یا کلروفیلPhotosystem - سیستم عکسFerredoxin - فریدوکسیینPrompt fluorescence - فلوئورسانس فوریDelayed fluorescence - فلورسانس تاخیریReaction centre - مرکز واکنشPlastocyanin - پلاستوسیانینplastoquinol - پلاستوکینولplastoquinone - پلواستیکینونOxygen-evolving complex - پیچیده اکسیژن تکامل یافتهResurrection plants - گیاهان رستاخیز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A new instrument (M-PEA), which measures simultaneously kinetics of prompt fluorescence (PF), delayed fluorescence (DF) and modulated light reflection at 820 nm (MR), was used to screen dark-adapted leaves of the resurrection plant Haberlea rhodopensis during their progressive drying, down to 1% relative water content (RWC), and after their re-watering. This is the first investigation using M-PEA, which employs alternations of actinic light (627-nm peak, 5000 μmol photons mâ 2 sâ 1) and dark intervals, where PF-MR and DF kinetics are respectively recorded, with the added advantages: (a) all kinetics are recorded with high time resolution (starting from 0.01 ms), (b) the dark intervals' duration can be as short as 0.1 ms, (c) actinic illumination can be interrupted at different times during the PF transient (recorded up to 300 s), with the earliest interruption at 0.3 ms. Analysis of the simultaneous measurements at different water-content-states of H. rhodopensis leaves allowed the comparison and correlation of complementary information on the structure/function of the photosynthetic machinery, which is not destroyed but only inactivated (reversibly) at different degrees; the comparison and correlation helped also to test current interpretations of each signal and advance their understanding. Our results suggest that the desiccation tolerance of the photosynthetic machinery in H. rhodopensis is mainly based on mechanism(s) that lead to inactivation of photosystem II reaction centres (transformation to heat sinks), triggered already by a small RWC decrease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1797, Issues 6â7, JuneâJuly 2010, Pages 1313-1326
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1797, Issues 6â7, JuneâJuly 2010, Pages 1313-1326
نویسندگان
Reto J. Strasser, Merope Tsimilli-Michael, Sheng Qiang, Vasilij Goltsev,