کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8299050 | 1537050 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Export or recombination of charges in reaction centers in intact cells of photosynthetic bacteria
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کلمات کلیدی
ubiquinoneBChlbacteriochlorophyll dimerbacteriochlorophyll - باکتری کلروفیوRedox Chemistry - ردوکس شیمیcyt - سیتcytochrome - سیتوکرومPhotosynthesis - فتوسنتزDelayed fluorescence - فلورسانس تاخیریLight harvesting complex - مجتمع برداشت نورreaction center - مرکز واکنشreaction center protein - پروتئین مرکز واکنشQuinone - کینون primary quinone acceptor - گیرنده اولیه کینونsecondary quinone acceptor - گیرنده ثانویه کوینون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Export or recombination of charges in reaction centers in intact cells of photosynthetic bacteria Export or recombination of charges in reaction centers in intact cells of photosynthetic bacteria](/preview/png/8299050.png)
چکیده انگلیسی
The kinetics and thermodynamics of forward and reverse electron transfer around the reaction center of purple bacterium Rhodobacter sphaeroides were studied in vivo by flash-excited delayed fluorescence, prompt fluorescence (induction) and kinetic difference absorption. By protection of the photomultiplier from intense bacteriochlorophyll prompt fluorescence evoked by laser excitation, the time resolution of the fluorometer was reduced typically 10 μs. Two precursor states of the delayed fluorescence were identified: P+QAâ and cyt c23+QAâ whose enthalpy levels were 340 meV and 1020 meV below Aâ, respectively. The free energy of the P+QAâ state relative to Aâ was â 870 meV in whole cells. Similar values were obtained earlier for isolated reaction center and chromatophore. The free energies of cyt c23+QAâ and P+QAâ states showed no or very weak (â 6 meV/pH unit) pH-dependence, respectively, supporting the concept of pH-independent redox midpoint potential of QA/QAâ in intact cells. In accordance with the multiphasic kinetics of delayed fluorescence, the kinetics of re-opening of the closed reaction center is also complex (it extends up to 1 s) as a consequence of acceptor and donor-side reactions. The control of charge export from the reaction center by light regime, redox agents and inhibitors is investigated. The complex kinetics may arise from the distribution of quinones in different redox states on the acceptor side (QB binding site and pool) and from organization of electron transfer components in supercomplexes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1787, Issue 12, December 2009, Pages 1444-1450
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1787, Issue 12, December 2009, Pages 1444-1450
نویسندگان
Emese Asztalos, Péter Maróti,