کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8301344 | 1537678 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prep1 deficiency improves metabolic response in white adipose tissue
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کلمات کلیدی
hK1Prep1TNFalphaFABP4PPARgammaIFNgammaMIP-1MCP1PKBPEPCKGLUT4C/EBP - C / EBPAdipokine - آدپیکینAdipogenesis - آدیپوژنزAdipose tissue - بافت چربیtumor necrosis factor alpha - تومور نکروز عامل آلفاglucose transporter 4 - حمل کننده گلوکز 4cluster of differentiation - خوشه تمایزInsulin signaling - سیگنالینگ انسولینphosphoenolpyruvate carboxykinase - فسفوآنولپیرود کربوکسیکینازhexokinase 1 - هگزوکیناز 1monocyte chemotactic protein 1 - پروتئین chemotactic monocyte 1CCAAT/enhancer binding protein - پروتئین اتصال CCAAT / enhancerfatty acid binding protein 4 - پروتئین اتصال دهنده اسید چرب 4macrophage inflammatory protein - پروتئین التهابی ماکروفاژprotein kinase B - پروتئین کیناز BInterferon gamma - گاما اینترفرونPeroxisome proliferator-activated receptor gamma - گاما گیرنده گیرنده فعال پرولیفیزوم فعالinsulin receptor - گیرنده انسولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Prep1 is a gene encoding for a homeodomain transcription factor which induces hepatic and muscular insulin resistance. In this study, we show that Prep1 hypomorphic heterozygous (Prep1i/+) mice, expressing low levels of protein, featured a 23% and a 25% reduction of total body lipid content and epididymal fat, respectively. The percentage of the small adipocytes (25-75â¯Î¼m) was 30% higher in Prep1i/+ animals than in the WT, with a reciprocal difference in the large adipose cells (100-150 and >150â¯Î¼m). Insulin-stimulated insulin receptor tyrosine and Akt serine phosphorylation markedly increased in Prep1i/+ mice, paralleled by 3-fold higher glucose uptake and a significant increase of proadipogenic genes such as C/EBPα, GLUT4, and FABP4. Moreover, T cells infiltration and TNF-α, IFNγ and leptin expression were reduced in adipose tissue from Prep1i/+ mice, while adiponectin levels were 2-fold higher. Furthermore, Prep1i/+ mature adipocytes released lower amounts of pro-inflammatory cytokines and higher amount of adiponectin compared to WT cells. Incubation of murine liver cell line (NMuLi) with conditioned media (CM) from mature adipocytes of Prep1i/+ mice improved glucose metabolism, while those from WT mice had no effect. Consistent with these data, Prep1 overexpression in 3T3-L1 adipocytes impaired adipogenesis and insulin signaling, and increased proinflammatory cytokine secretion. All these findings suggest that Prep1 silencing reduces inflammatory response and increases insulin sensitivity in adipose tissue. In addition, CM from mature adipocytes of Prep1i/+ mice improve metabolism in hepatic cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1863, Issue 5, May 2018, Pages 515-525
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1863, Issue 5, May 2018, Pages 515-525
نویسندگان
Antonietta Liotti, Serena Cabaro, Ilaria Cimmino, Serena Ricci, Claudio Procaccini, Orlando Paciello, Gregory A. Raciti, Rosa Spinelli, Susanna Iossa, Giuseppe Matarese, Claudia Miele, Pietro Formisano, Francesco Beguinot, Francesco Oriente,