کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8301569 | 1537701 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Whole-body DHA synthesis-secretion kinetics from plasma eicosapentaenoic acid and alpha-linolenic acid in the free-living rat
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کلمات کلیدی
SDATLELNAGC-FIDALALCPUFAtPATHASmaxDPAn-3n − 3tetracosahexaenoic acidt1/2 - t1 / 2α-linolenic acid - اسید α-لینولنیکArachidonic acid - اسید آراشیدونیکStearidonic acid - اسید استاریدونیdocosahexaenoic acid - اسید داکوزاگزوائونیکLinoleic acid - اسید لینولئیکPolyunsaturated fatty acid - اسید چرب غیر اشباعLong chain polyunsaturated fatty acid - اسید چرب چرب اشباع نشدهOmega-3 - امگا 3Gas chromatography-flame ionization detection - تشخیص یونیزاسیون گاز کروماتوگرافی-شعلهNutrition - تغذیهARA - در حال حاضرDHA - دوکوساهگزائنوئیک اسیدMetabolism - متابولیسم Fatty acid methyl ester - متیل استر اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربTurnover rate - نرخ گردشLipids - چربیهاtotal lipid extract - کل عصاره چربی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Whole body docosahexaenoic acid (DHA, 22:6n-3) synthesis from α-linolenic acid (ALA, 18:3n-3) is considered to be very low, however, the daily synthesis-secretion of DHA may be sufficient to supply the adult brain. The current study aims to assess whether whole body DHA synthesis-secretion kinetics are different when comparing plasma ALA versus eicosapentaenoic acid (EPA, 20:5n-3) as the precursor. Male Long Evans rats (n = 6) were fed a 2% ALA in total fat diet for eight weeks, followed by surgery to implant a catheter into each of the jugular vein and carotid artery and 3 h of steady-state infusion with a known amount of 2H-ALA and 13C-eicosapentaenoic acid (EPA, 20:5n3). Blood samples were collected at thirty-minute intervals and plasma enrichment of 2H- and 13C EPA, n â 3 docosapentaenoic acid (DPAn-3, 22:5n-3) and DHA were determined for assessment of synthesis-secretion kinetic parameters. Results indicate a 13-fold higher synthesis-secretion coefficient for DHA from EPA as compared to ALA. However, after correcting for the 6.6 fold higher endogenous plasma ALA concentration, no significant differences in daily synthesis-secretion (nmol/day) of DHA (97.6 ± 28.2 and 172 ± 62), DPAn-3 (853 ± 279 and 1139 ± 484) or EPA (1587 ± 592 and 1628 ± 366) were observed from plasma unesterified ALA and EPA sources, respectively. These results suggest that typical diets which are significantly higher in ALA compared to EPA yield similar daily DHA synthesis-secretion despite a significantly higher synthesis-secretion coefficient from EPA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1861, Issue 9, Part A, September 2016, Pages 997-1004
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1861, Issue 9, Part A, September 2016, Pages 997-1004
نویسندگان
Adam H. Metherel, Anthony F. Domenichiello, Alex P. Kitson, Kathryn E. Hopperton, Richard P. Bazinet,