کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8302130 | 1537721 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The mannoprotein TIR3 (CAGL0C03872g) is required for sterol uptake in Candida glabrata
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کلمات کلیدی
GPicomplete synthetic mediumYPdSterol uptakeUPC27-DehydrocholesterolCandida glabrataCSMRPMIRT-PCRMICGFPORF7-DHC - 7 DHCMinimum inhibitory concentration - حداقل غلظت مهاریopen reading frame - قاب خواندن بازMannoprotein - مانور پروتئینRoswell Park Memorial Institute medium - موسسه خاطرات Roswell Park mediumhemagglutinin - هماگلوتینینReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایgreen fluorescent protein - پروتئین فلورسنت سبزhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراglycosylphosphatidylinositol - گلیکوزیل فسفاتیدیلینوزیتول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The mannoprotein TIR3 (CAGL0C03872g) is required for sterol uptake in Candida glabrata The mannoprotein TIR3 (CAGL0C03872g) is required for sterol uptake in Candida glabrata](/preview/png/8302130.png)
چکیده انگلیسی
Sterol uptake in the pathogenic fungus, Candida glabrata, occurs via the sterol transporter, CgAus1p. Azole inhibition of sterol biosynthesis can under certain circumstances be reversed by adding exogenously sterol. Here we demonstrate that the CgTIR3 (CAGL0C03872g) gene product is also required for sterol uptake, since Cgtir3Î strains fail to take up sterol both aerobically and under hypoxic conditions. Western analysis using an HA-tagged TIR3 strain showed that CgTir3p localizes to the cell wall, and its expression is induced by serum. Semi-quantitative reverse transcriptase-PCR also showed that two transcription regulatory genes, CgUPC2A and CgUPC2B, control CgTIR3 as well as CgAUS1 gene expression. Interestingly, complementation studies using Cgtir3Î showed that ScDAN1, a mannoprotein required for sterol uptake in Saccharomyces cerevisiae, could not complement the C. glabrata TIR3 function. Furthermore, sterol analyses, in which both the CgAUS1 and CgTIR3 genes were constitutively expressed, resulted in aerobic sterol uptake although the amount of uptake was considerably less than that of cells cultured aerobically with serum. These results suggest that additional factors other than CgAUS1 and CgTIR3 are required for sterol uptake in C. glabrata.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 2, February 2015, Pages 141-151
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 2, February 2015, Pages 141-151
نویسندگان
Tatsuya Inukai, Minoru Nagi, Akihiro Morita, Koichi Tanabe, Toshihiro Aoyama, Yoshitsugu Miyazaki, Martin Bard, Hironobu Nakayama,