کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8303699 | 1537949 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Junctional adhesion molecule C (JAM-C) dimerization aids cancer cell migration and metastasis
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کلمات کلیدی
PDZzinc finger E-box-binding homeobox 1lymphoid enhancer-binding factor 1LEF-1ZEB1ECMEGFMMPTight junction - اتصال تنگepithelial to mesenchymal transition - اپیتلیال به انتقال مزانشیمالEMT - تکنسین فوریتهای پزشکیTnT - تی ان تیTumor cell - سلول تومورEpithelial growth factor - عامل رشد اپیتلیالExtracellular matrix - ماتریکس خارج سلولیmatrix metalloproteinase - ماتریکس متالوپروتئینازMetastasis - متاستاز JAM - مرباMigration - مهاجرتMutagenesis - موتاژنزjunctional adhesion molecule - مولکول چسبندگی مجاورAdhesion - چسبندگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Junctional adhesion molecule C (JAM-C) dimerization aids cancer cell migration and metastasis Junctional adhesion molecule C (JAM-C) dimerization aids cancer cell migration and metastasis](/preview/png/8303699.png)
چکیده انگلیسی
Most cancer deaths result from metastasis, which is the dissemination of cells from a primary tumor to distant organs. Metastasis involves changes to molecules that are essential for tumor cell adhesion to the extracellular matrix and to endothelial cells. Junctional Adhesion Molecule C (JAM-C) localizes at intercellular junctions as homodimers or more affine heterodimers with JAM-B. We previously showed that the homodimerization site (E66) in JAM-C is also involved in JAM-B binding. Here we show that neoexpression of JAM-C in a JAM-C-negative carcinoma cell line induced loss of adhesive property and pro-metastatic capacities. We also identify two critical structural sites (E66 and K68) for JAM-C/JAM-B interaction by directed mutagenesis of JAM-C and studied their implication on tumor cell behavior. JAM-C mutants did not bind to JAM-B or localize correctly to junctions. Moreover, mutated JAM-C proteins increased adhesion and reduced proliferation and migration of lung carcinoma cell lines. Carcinoma cells expressing mutant JAM-C grew slower than with JAM-C WT and were not able to establish metastatic lung nodules in mice. Overall these data demonstrate that the dimerization sites E66-K68 of JAM-C affected cell adhesion, polarization and migration and are essential for tumor cell metastasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1865, Issue 4, April 2018, Pages 638-649
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1865, Issue 4, April 2018, Pages 638-649
نویسندگان
Sarah Garrido-Urbani, Alain Vonlaufen, Jimmy Stalin, Maria De Grandis, Patricia Ropraz, Stéphane Jemelin, Florence Bardin, Holger Scheib, Michel Aurrand-Lions, Beat A. Imhof,