Genotyping for CYP2C9 and VKORC1 alleles by a novel point of care assay with HyBeacon® probes

► Knowledge of VKORC1 and CYP2C9 genotype is of value in setting initial coumarin anticoagulant dose. ► A rapid real-time PCR genotyping assay for VKORC1 and CYP2C9 is described. ► The assay uses whole blood and can be done at point of care by clinical staff. ► The assay was validated successfully in 156 individuals.