Method development for routine liquid chromatography-mass spectrometry measurement of the alcohol biomarker phosphatidylethanol (PEth) in blood

The LC-ESI-MS(/MS) method allowed for simultaneous qualitative and quantitative measurements of PEth forms in whole blood samples. Related to the routine application of blood PEth as alcohol biomarker, reference intervals were suggested for total PEth and the major molecular forms PEth-16:0/18:1 and 16:0/18:2.