کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8318913 | 1539158 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation of estrogen receptor subtypes and vitellogenin genes: Expression in female Chalcalburnus tarichi
ترجمه فارسی عنوان
جداسازی ژن های گیرنده استروژن و ژن های ویلیوگنین: بیان در زن چالکبالونوس تارچی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Reproductively arrested gonadal development has been previously described in the teleost pearl mullet (Chalcalburnus tarichi, Cyprinidae) from Van Edremit Gulf of Lake Van, Turkey. Oocyte development in some females was arrested at the previtellogenic stage, while gonadosomatic index (GSI) and plasma 17β-estradiol (E2) level were low. A subset of the females was found to have normal ovaries and relatively higher plasma E2 and GSI. These two groups were termed reproductively arrested (RA) and reproductively non-arrested (RN) females. In this study, we cloned estrogen receptor (ER) isoforms (ERα, ERβ1 and ERβ2) and vitellogenin (Vtg), and their mRNA levels were measured in RA and RN fish tissues. C. tarichi ERs fell in the same clade with other fish ERs and ERα and ERβ1 had 97% and 98% identity with the roach (Rutilus rutilus) ERs, respectively. Both Vtg and ER isoforms' mRNA abundance were higher in the liver than in the ovary and hypothalamus (liver > ovary > hypothalamus). The level of ERα mRNA was significantly lower in the liver, ovary and brain of RA fish than in the RN fish tissues. ERβ1 mRNA levels were not different in the liver and ovary from RA and RN fish while ERβ2 expression significantly increased in the liver and ovary from RA fish. All ER subtype expression was found to be lower in the brain from RA fish than RN fish. The level of Vtg mRNA was significantly lower in the liver and ovary from RA fish than RN fish tissue. These results suggest that ER subtypes are differentially regulated by E2, and their functions are also different in vitellogenesis. Analysis of organic contaminants in sediments revealed that C. tarichi living in Van Edremit Gulf of Lake Van are exposed to the contaminants bis(2-ethylhexyl) phthalate and 4,4ⲠDDT. We suggest that the RA fish represent a segment of the population that is more sensitive to exposure to endocrine disrupting compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volumes 172â173, JuneâJuly 2014, Pages 67-73
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volumes 172â173, JuneâJuly 2014, Pages 67-73
نویسندگان
Guler Unal, Emily C. Marquez, Mara Feld, Pericles Stavropoulos, Ian P. Callard,