Regulation of the bacteriophage T4 Dda helicase by Gp32 single-stranded DNA-binding protein

- Stimulation of DNA synthesis by Dda helicase requires Gp32 ssDNA-binding protein.
- Gp32-Dda protein-protein interactions stimulate Dda helicase and ATPase activities.
- Gp32 strongly inhibits unwinding of inverted replication fork structures by Dda.
- Gp32 controls where Dda may assemble on DNA replication and repair intermediates.
- Gp32 regulation of Dda may be important for the mechanism of replication restart.