کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8321932 | 1539842 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PaDef defensin from avocado (Persea americana var. drymifolia) is cytotoxic to K562 chronic myeloid leukemia cells through extrinsic apoptosis
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Plant defensins, a group of antimicrobial peptides, show selective cytotoxicity toward cancer cells. However, their mechanisms of action remain poorly understood. Here, we evaluated the cytotoxicity of PaDef defensin from avocado (Persea americana var. drymifolia) on K562 chronic myeloid leukemia cells and analyzed the pathway involved in the induction of cell death. The defensin PaDef was not cytotoxic against human PBMCs; however, it was cytotoxic for K562 cell line (IC50â¯=â¯97.3â¯Î¼g/ml) activating apoptosis at 12â¯h. PaDef did not affect the mitochondrial membrane potential (ÎΨm), neither the transmembranal potential or the release of intracellular calcium. Also, PaDef induced gene expression of caspase 8 (â¼2 fold), TNF-α (â¼4 fold) and TNFR1 (â¼10 fold). In addition, the activation of caspase 8 was detected at 24â¯h, whereas caspase 9 activity was not modified, suggesting that the extrinsic apoptosis pathway could be activated. In conclusion, PaDef induces apoptosis on K562 cells, which is related to the activation of caspase 8 and involves the participation of TNF-α, which is a novel property for a plant defensin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 99, June 2018, Pages 10-18
Journal: The International Journal of Biochemistry & Cell Biology - Volume 99, June 2018, Pages 10-18
نویسندگان
Luis José Flores-Alvarez, Jaquelina Julia Guzmán-RodrÃguez, Rodolfo López-Gómez, Rafael Salgado-Garciglia, Alejandra Ochoa-Zarzosa, Joel E. López-Meza,