کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322380 | 1539874 | 2016 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Autophagic lysosomal reformation depends on mTOR reactivation in H2O2-induced autophagy
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کلمات کلیدی
DMEMPI4PPIP5KALREBSSATGRab7HBSSmTORDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoPhosphatidylinositol 4-phosphate - Phosphatidylinositol 4-فسفاتPI(4,5)P2 - PI (4،5) P2Hydrogen peroxide - آب اکسیژنهAutophagy - اتوفاژیPhosphatidylinositol 4-phosphate 5-kinase - فسفاتیدیلینواستیل 4-فسفات 5-کینازHank's buffered salt solution - محلول نمک بافر هانکH2O2 - هیدروژن پراکسیدautophagy-related gene - ژن مربوط به autophagyStarvation - گرسنگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Autophagic lysosomal reformation, a key cellular process for maintaining lysosome homeostasis in elevated autophagy, so far has only been reported for cells under certain forms of starvation. For this reason, it is controversial that whether this phenomenon is starvation-specific and its importance in lysosomal regeneration at the late stage of autophagy is often challenged. Here we show that exogenous hydrogen peroxide (H2O2) induced lysosome depletion and recovery characteristic of autophagic lysosomal reformation, and we confirmed the occurrence of autophagic lysosomal reformation after H2O2 treatment by demonstrating Rab7 dissociation from autolysosomes, recruitment of Phosphatidylinositol 4-phosphate (PI4P) and clathrin to the surface of autolysosomes, and the existence of tubular “pro-lysosome” structures extending from autolysosomes. Similar to starvation, H2O2 caused an initial deactivation and a subsequent reactivation for mTOR, and mTOR reactivation was essential for ALR. Our results provided a first non-starvation example of autophagic lysosomal reformation and provide evidence for its importance for some autophagic processes other than that of starvation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 70, January 2016, Pages 76-81
Journal: The International Journal of Biochemistry & Cell Biology - Volume 70, January 2016, Pages 76-81
نویسندگان
Jiqian Zhang, Wei Zhou, Jun Lin, Pengfei Wei, Yunjiao Zhang, Peipei Jin, Ming Chen, Na Man, Longping Wen,