کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322826 | 1539884 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glyceraldehyde-3-phosphate dehydrogenase is required for efficient repair of cytotoxic DNA lesions in Escherichia coli
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کلمات کلیدی
GPHSSBMMSBERExoCFUsExonucleaseGAPDHGSTUDGIPTGNTACAANIRUracil DNA glycosylase - DNA گلیکوزیلاز اوراسیلEndonuclease - آندونوکراسیEndonuclease IV - آندونوکراسی IVnitrilotriacetic acid - اسید نیتریلوتوریکاتیکendo - اندوisopropyl β-D-1-thiogalactopyranoside - ایزوپروپیل β-D-1-thiogalactopyranosideBleomycin - بلئومایسینDNA repair - ترمیم DNAbase excision repair - تعمیر پایه پایهNucleotide Incision Repair - تعمیرات برش ناکولوژیکRifampicin - ریفامپینAP sites - سایت های APluria-bertani broth - سوپ لووریا برتانیPhosphoglycolate phosphatase - فسفوگلیکات فسفاتازminimal medium - متوسط متوسطmethyl methanesulfonate - متیل متان سولفوناتHomologous recombination - نوترکیبی همولوگcolony-forming units - واحدهای تشکیل دهنده کلنیSingle-stranded DNA binding protein - پروتئین متصل به یک رشته DNARif - کد عکسglutathione-S-transferase - گلوتاتیون S-ترانسفرازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein with diverse biological functions in human cells. In bacteria, moonlighting GAPDH functions have only been described for the secreted protein in pathogens or probiotics. At the intracellular level, we previously reported the interaction of Escherichia coli GAPDH with phosphoglycolate phosphatase, a protein involved in the metabolism of the DNA repair product 2-phosphoglycolate, thus suggesting a putative role of GAPDH in DNA repair processes. Here, we provide evidence that GAPDH is required for the efficient repair of DNA lesions in E. coli. We show that GAPDH-deficient cells are more sensitive to bleomycin or methyl methanesulfonate. In cells challenged with these genotoxic agents, GAPDH deficiency results in reduced cell viability and filamentous growth. In addition, the gapA knockout mutant accumulates a higher number of spontaneous abasic sites and displays higher spontaneous mutation frequencies than the parental strain. Pull-down experiments in different genetic backgrounds show interaction between GAPDH and enzymes of the base excision repair pathway, namely the AP-endonuclease Endo IV and uracil DNA glycosylase. This finding suggests that GAPDH is a component of a protein complex dedicated to the maintenance of genomic DNA integrity. Our results also show interaction of GAPDH with the single-stranded DNA binding protein. This interaction may recruit GAPDH to the repair sites and implicates GAPDH in DNA repair pathways activated by profuse DNA damage, such as homologous recombination or the SOS response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 60, March 2015, Pages 202-212
Journal: The International Journal of Biochemistry & Cell Biology - Volume 60, March 2015, Pages 202-212
نویسندگان
Elaine Ferreira, Rosa Giménez, MarÃa Alexandra Cañas, Laura Aguilera, Juan Aguilar, Josefa Badia, Laura Baldomà ,