کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322886 | 1539887 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel centrosome and microtubules associated subcellular localization of Nogo-A: Implications for neuronal development
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کلمات کلیدی
DRGNeuro2aNogo-AVPAMTOCSprague-Dawley - اسپراگ داولیUndifferentiated - بی تفاوتیneuronal development - توسعه عصبیCNS - دستگاه عصبی مرکزیCentrosome - سانتریفیوژcentral nervous system - سیستم عصبی مرکزیmicrotubule organizing center - مرکز برگزاری میکروتوبول هاMicrotubules - میکروتوبول، ریزلوله هاValproic acid - والپروات و والپروات سدیم یا والپروئیک اسیدdorsal root ganglia - گانگلیس ریشه پشتی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel centrosome and microtubules associated subcellular localization of Nogo-A: Implications for neuronal development A novel centrosome and microtubules associated subcellular localization of Nogo-A: Implications for neuronal development](/preview/png/8322886.png)
چکیده انگلیسی
Oligodendrocyte-derived neurite-outgrowth inhibitor Nogo-A and its restriction mechanism are well-known. Recently, Nogo-A is reported to be abundantly expressed in neurons, however, the concrete link between neuronal Nogo-A and neuronal development is poorly understood. In the present study, we used Neuro2A and COS7 cell lines to clarify that Nogo-A largely distributed in the centrosome and microtubules-rich regions. When endogenous Nogo-A was down-regulated with RNA interference, the percentage of cell differentiation and the total neurite length of Neuro2A exposed to valproic acid (VPA) decreased sharply. Furthermore, in primary neurons, acetylated α-tubulin decreased at the tips of neurites where endogenous Nogo-A was still highly expressed. In HEK293FT cell lines, Nogo-A overexpression could redistribute acetylated α-tubulin but not change the level of α-tubulin. Together, our data discovered that centrosome- and microtubules-localized Nogo-A positively regulates neuronal differentiation and neurite outgrowth of Neuro2A cell lines, implicating the essential roles of subcellular Nogo-A in neuronal development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 57, December 2014, Pages 1-6
Journal: The International Journal of Biochemistry & Cell Biology - Volume 57, December 2014, Pages 1-6
نویسندگان
Yajing Mi, Xingchun Gao, Yue Ma, Jie Gao, Zhen Wang, Weilin Jin,