کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323383 | 1539891 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Disialoganglioside GD3-synthase over expression inhibits survival and angiogenesis of pancreatic cancer cells through cell cycle arrest at S-phase and disruption of integrin-β1-mediated anchorage
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کلمات کلیدی
MAAMaackia amurensis lectinSambucus nigra lectinGD1ab-FGF4-methylumbelliferoneHIF1αGD3MUACeIF4EFGFRECMSNADAPINeu5AcFACSHMECMFIFITCEGFEGFR3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromide4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولeukaryotic translation initiation factor 4E - 4e عامل آغازگر ترجمه یوکاریوتیiGFR - IGFRMTT - MTTPancreatic ductal adenocarcinoma - آدنوکارسینوم پانکراس داکتالPDAC یا pancreatic ductal adenocarcinoma - آدنوکارسینومای داکتال پانکراسAngiogenesis - آنژیوژنزhuman microvascular endothelial cell - سلول اندوتلیال میکرواسکولی انسانHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانSialyltransferase - سیالیال ترانسفرازhypoxia-inducible factor 1-alpha - عامل القایی هیپوکسی 1-آلفاepidermal growth factor - عامل رشد اپیدرمیS phase - فاز SVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)basic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتfluorescence-activated cell sorter - فلورسانس فعال سلول مرتب سازextra-cellular matrix - ماتریکس خارج سلولیmean fluorescence intensity - میانگین شدت فلورسانسEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمالfibroblast growth factor receptor - گیرنده فاکتور رشد فیبروبلاستInsulin-like growth factor receptor - گیرنده فاکتور رشد مانند انسولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gangliosides play important roles in the development, differentiation and proliferation of mammalian cells. They bind to other cell membrane components through their terminal sialic acids. Different gangliosides influence cellular functions based on the positions and linkages of sialic acids. Expression of gangliosides mainly depends on the status of sialic acid-modulatory enzymes, such as different types of sialyltransferases and sialidases. One such sialyltransferase, disialoganglioside GD3 synthase, is specifically responsible for the production of GD3. Pancreatic ductal adenocarcinoma, making up more than 90% of pancreatic cancers, is a fatal malignancy with poor prognosis. Despite higher sialylation status, the disialoganglioside GD3 level is very low in this cancer. However, the exact status and function of this disialoganglioside is still unknown. Here, we intended to study the intracellular mechanism of disialoganglioside GD3-induced apoptosis and its correlation with the adhesion and angiogenic pathways in pancreatic cancer. We demonstrated that disialoganglioside GD3 synthase-transfected cells showed enhanced apoptosis and it caused the arrest of these cells in the S-phase of the cell cycle. Integrins, a family of transmembrane proteins play important role in cell-cell recognition, invasion, adhesion and migration. disialoganglioside GD3 co-localised with integrin-β1 and thereby inhibited it's downstream signalling in transfected cells. Transfected cells exhibited inhibition of cell adhesion with extracellular matrix proteins. Enhanced GD3 expression down regulated angiogenesis-regulatory proteins and inhibited epidermal growth factor/vascular endothelial growth factor-driven angiogenic cell growth in these cells. Taken together, our study provides support for the GD3-induced cell cycle arrest, disruption of integrin-β1-mediated anchorage, inhibition of angiogenesis and thereby induced apoptosis in pancreatic cancer cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 53, August 2014, Pages 162-173
Journal: The International Journal of Biochemistry & Cell Biology - Volume 53, August 2014, Pages 162-173
نویسندگان
Chandan Mandal, Sayantani Sarkar, Uttara Chatterjee, Reinhard Schwartz-Albiez, Chitra Mandal,