کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323777 | 1539898 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytokines secreted by macrophages isolated from tumor microenvironment of inflammatory breast cancer patients possess chemotactic properties
ترجمه فارسی عنوان
سیتوکین های ترشح شده توسط ماکروفاژها جدا شده از محیط میکروسکوپی تومور در بیماران مبتلا به سرطان التهابی دارای خواص شیمیایی
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کلمات کلیدی
IL-10c-fmsTAMsINF-γIL-13CCL-2HCMVIL-8C-C chemokine receptor type 2CCR2STAT3MMP-2MCP-1MMP-9NF-κBPI3KIBCIL-4interferon-γ - اینترفرون-γinterleukin-4 - اینترلوکین -4Interleukin-10 - اینترلوکین 10Interleukin-13 - اینترلوکین 13Motility - تحرکInvasion - تهاجمTumor associated macrophages - تومور مرتبط با ماکروفاژهاtumor necrosis factor-alpha - تومور نکروز عامل آلفاInflammatory breast cancer - سرطان پستان التهابیHuman cytomegalovirus - سیتومگالوویروس انسانیCytokines - سیتوکین هاTNF-α - فاکتور نکروز توموری آلفاnuclear factor kappa beta - فاکتور هسته ای کاپا بتاmatrix metalloproteinases-2 - ماتریکس فلزوپروتئیناز-2matrix metalloproteinases-9 - ماتریکس متالوپروتئیناز -9Macrophages - ماکروفاژها،درشت خوارهاAlternatively activated macrophages - ماکروفاژهای فعال متناوبSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیmonocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Although there is a growing literature describing the role of macrophages in breast cancer, the role of macrophages in inflammatory breast cancer (IBC) is unclear. The aim of present study was to isolate and characterize tumor associated macrophages of IBC and non-IBC patients and define their role in IBC. Tumor infiltrating monocytes/macrophages (CD14+ and CD68+) were measured by immunohistochemistry using specific monoclonal antibodies. Blood drained from axillary vein tributaries was collected during breast cancer surgery and the percentage of CD14+ in the total isolated leukocytes was assessed by flow cytometric analysis. CD14+ cells were separated from total leukocytes by immuno-magnetic beads technique and were cultured overnight. Media conditioned by CD14+ were collected and subjected to cytokine profiling using cytokine antibody array. Wound healing and invasion assays were used to test whether cytokines highly secreted by tumor drained macrophages induce motility and invasion of breast cancer cells. We found that macrophages highly infiltrate into carcinoma tissues of IBC patients. In addition blood collected from axillary tributaries of IBC patients is highly enriched with CD14+ cells as compared to blood collected from non-IBC patients. Cytokine profiling of CD14+ cells isolated from IBC patients revealed a significant increase in secretion of tumor necrosis factor-α; monocyte chemoattractant protein-1/CC-chemokine ligand 2; interleukin-8 and interleukin-10 as compared to CD14+ cells isolated from non-IBC patients. Tumor necrosis factor-α, interleukin-8 and interleukin-10 significantly increased motility and invasion of IBC cells in vitro. In conclusion, macrophages isolated from the tumor microenvironment of IBC patients secrete chemotactic cytokines that may augment dissemination and metastasis of IBC carcinoma cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 46, January 2014, Pages 138-147
Journal: The International Journal of Biochemistry & Cell Biology - Volume 46, January 2014, Pages 138-147
نویسندگان
Mona M. Mohamed, Eslam A. El-Ghonaimy, Mohamed A. Nouh, Robert J. Schneider, Bonnie F. Sloane, Mohamed El-Shinawi,