کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8324775 | 1539922 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNA methylation and histone modifications modulate the β1,3 galactosyltransferase β3Gal-T5 native promoter in cancer cells
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کلمات کلیدی
TSA5AzaNF-Y5-aza-2′deoxycytidineLTRcarbohydrate antigen - آنتی ژن کربوهیدراتchromatin immunoprecipitation - ایمن سازی کروماتینGene expression - بیان ژنTrichostatin A - تریکوستاتین ALong terminal repeat - تکرار طولانی مدتreverse transcription - رونویسی معکوسTumor marker - نشانگر تومورHistone 3 - هیستون 3CHiP - چیپGalactosyltransferase - گالاکتوزیلتransferaseGlycobiology - گلیکوبیولوژیGlycosyltransferase - گلیکوزیلتransferase
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The native promoter of β1,3 galactosyltransferase β3Gal-T5 contributes to the expression of the enzyme and its oligosaccharide products, such as Lewis antigens, in many tissues. It is mainly sensitive to nuclear factor NF-Y and located nearby two CpG islands. To elucidate the regulation of the native promoter, we analyzed NF-Y protein and β3Gal-T5 mRNA, and found that NF-Y is scarcely modulated among various cell lines and biopsies from normal or cancerous colon. Conversely, β3Gal-T5 expression levels vary in the cell lines and are strongly down-regulated in colon cancer. We also performed quantitative methylation analysis of β3Gal-T5 CpG islands and found an inverse correlation between mRNA expression and DNA methylation. In particular, the methylation levels of both islands are always increased in cancer, with respect to the corresponding normal counterpart, in matched normal and tumor samples of colon and breast origin. Moreover, treatment with chromatin remodeling agents 5-aza-2â²deoxycytidine and trichostatin A does not restore transcription in completely negative cells, but only increases expression in basally positive cells. However, methylation analysis after 5-aza-2â²deoxycytidine treatment revealed partial demethylation of both islands in all treated cells. Finally, chromatin immunoprecipitation assays on β3Gal-T5 promoter showed that histone H3K4 trymethylation, H3K79 dimethylation, and H3K9-14 acetylation are high in cells expressing the transcript, and very low in those negative, while H4K20 trimethylation and H3K27 dimethylation are the opposite. We conclude that complex epigenetic modulation underlies the regulation of β3Gal-T5 native promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 44, Issue 1, January 2012, Pages 84-90
Journal: The International Journal of Biochemistry & Cell Biology - Volume 44, Issue 1, January 2012, Pages 84-90
نویسندگان
Anna Caretti, Silvia M. Sirchia, Silvia Tabano, Aida Zulueta, Fabio Dall'Olio, Marco Trinchera,