کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326278 | 1539965 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Diabetes induces changes of catecholamines in primary mesangial cells
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کلمات کلیدی
DβHnonobese diabetic micetetrahydrobiopterinBH4MAOEPINODddC - DDCdopa decarboxylase - dopa dearboxylaseEpinephrine - اپی نفرینDiabetes - بیماری قندtyrosine hydroxylase - تیروزین هیدروکسیلازDopamine - دوپامینdopamine β-hydroxylase - دوپامین β-هیدروکسیلازMesangial cell - سلول ماندانژیmesangial cells - سلول های مزانژیSwiss mice - موش سوئیسیmonoamine oxidase - مونوآمین اکسیدازها norepinephrine - نوراپی نفرینcatecholamine - کاتکولآمینهاhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Diabetes mellitus is a frequent cause of kidney function damage with diabetic nephropathy being predominantly related to glomerular dysfunction. Diabetes is capable of interfering with distinct hormonal systems, as well as catecholamine metabolism. Since mesangial cells, the major constituent of renal glomerulus, constitute a potential site for catecholamine production, the present study was carried out to investigate alterations in catecholamine metabolism in cultured mesangial cells from the nonobese diabetic mouse, a well-established model for type I diabetes. We evaluated mesangial cells from normoglycemic and hyperglycemic nonobese diabetic mice, as well as cells from normoglycemic Swiss mice as control. Mesangial cells from normoglycemic mice presented similar profiles concerning all determinations. However, cells isolated from hyperglycemic animals presented increased dopamine and norepinephrine production/secretion. Among the studied mechanisms, we observed an upregulation of tyrosine hydroxylase expression accompanied by increased tetrahydrobiopterin consumption, the tyrosine hydroxylase enzymatic cofactor. However, this increase in synthetic pathways was followed by decreased monoamine oxidase activity, which corresponds to the major metabolic pathway of catecholamines in mesangial cells. In addition, whole kidney homogenates from diabetic animals also presented increased dopamine and norepinephrine levels when compared to normoglycemic animals. Thus, our results suggest that diabetes alters catecholamine production by interfering with both synthesizing and degrading enzymes, suggesting a possible role of catecholamine in the pathogenesis of acute and chronic renal complications of diabetes mellitus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 40, Issue 4, 2008, Pages 747-754
Journal: The International Journal of Biochemistry & Cell Biology - Volume 40, Issue 4, 2008, Pages 747-754
نویسندگان
Giovana Seno Di Marco, Juliana Almada Colucci, Fernanda Barrinha Fernandes, Carlos Pablo Vio, Nestor Schor, Dulce Elena Casarini,