کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8334245 | 1540271 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improvement of antioxidant activity of peptides with molecular weights ranging from 1 to 10Â kDa by PEF technology
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کلمات کلیدی
BBDDPPHEWPPEF2,2-diphenyl-1-picrylhydrazyl - 2،2-دی فینیل-1-پریکیل هیدرازیلstandard deviation - انحراف معیارResponse surface methodology - روش سطح پاسخResponse surface methodology (RSM) - روششناسی سطح پاسخ RSM - روششناسی سطح پاسخBox-Behnken design - طرح جعبه BehnkenAntioxidant activity - فعالیت آنتیاکسیدانیPulsed electric field - میدان الکتریکی پالسیPulsed electric field (PEF) - میدان الکتریکی پالسی (PEF)Molecular weight - وزن مولکولیEgg white protein - پروتئین سفید تخم مرغ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Egg white protein powder was hydrolyzed with Alcalase to produce antioxidant peptides. Then, the peptides were fractionated with ultrafiltration membranes. The peptides (1-10 kDa) were further treated by pulsed electric field (PEF) to investigate its effect on the antioxidant activity of the peptides. Antioxidant activity was evaluated using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition assay. The results indicated that optimal electric field intensity and standing times of PEF can enhance the antioxidant activity of the peptides. Therefore, a Box-Behnken design (BBD) with three independent variables including concentration, electric field intensity and pulse frequency was used to establish the regression equation of second-order response surface. The optimal conditions were as follows: concentration 8 mg/ml, electric field intensity 10 kV/cm and pulse frequency 2400 Hz. Under these conditions, the peptides antioxidant activity was 62.64% ± 0.98%. The present study demonstrated that the antioxidant activity of peptides (1-10 kDa) could be improved using PEF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 51, Issue 3, October 2012, Pages 244-249
Journal: International Journal of Biological Macromolecules - Volume 51, Issue 3, October 2012, Pages 244-249
نویسندگان
Jia Wang, Ke Wang, Songyi Lin, Ping Zhao, Gregory Jones, Hung Trang, Jingbo Liu, Haiqing Ye,