کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8334269 1540270 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The N-terminus modulates human Caf1 activity, structural stability and aggregation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The N-terminus modulates human Caf1 activity, structural stability and aggregation
چکیده انگلیسی
Caf1 is a deadenylase component of the CCR4-Not complex. Here we found that the removal of the N-terminus resulted in a 30% decrease in human Caf1 (hCaf1) activity, but had no significant influence on main domain structure. The removal of the N-terminus led to a decrease in the thermal stability, while the existence of the N-terminus promoted hCaf1 thermal aggregation. Homology modeling indicated that the N-terminus had a potency to form a short α-helix interacted with the main domain. Thus the N-terminus played a role in modulating hCaf1 activity, stability and aggregation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 51, Issue 4, November 2012, Pages 497-503
نویسندگان
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