Equilibrium studies of cellulase aggregates in presence of ascorbic and boric acid

► Cellulase aggregates at 300 mM ascorbic and 10 mM boric acid are detected. ► Strong protein-protein interactions owing to aggregation may affect the equilibrium of many reactions in vivo. ► The molten globule state of cellulase at 90 mM ascorbic acid is investigated. ► Ascorbic acid being an anti-oxidant shows pro-oxidant nature under certain conditions.