کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8337967 | 1540973 | 2017 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role and mechanism of AMH in the regulation of Sertoli cells in mice
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Sertoli cells produce anti-Müllerian hormone (AMH), a glycoprotein belonging to the transforming growth factor-beta family. AMH mediates the regression of Müllerian ducts in the developing male fetus. However, the role of AMH in the regulation of primary Sertoli cells remains unclear. The present study was designed to investigate the effect of AMH on the viability and proliferation of Sertoli cells, with an additional focus on stem cell factor (SCF). Treatment of Sertoli cells with increasing concentrations of rh-AMH (0, 10, 50, 100, and 800Â ng/ml) for two days revealed that AMH, at high concentrations, increased apoptosis. These results were confirmed by a significant increase in Caspase-3 and Bax and a decrease in Bcl-2 protein and mRNA expression (PÂ <Â 0.01). Paradoxically, treatment with a low concentration of rh-AMH (10Â ng/ml), but not higher concentrations (50-800Â ng/ml), promoted Sertoli cell proliferation, which was verified by an increase in PCNA mRNA (PÂ <Â 0.05). Furthermore, only low concentrations of rh-AMH activated the non-canonical ERK signaling pathway. Similarly, low concentrations of rh-AMH (10-50Â ng/ml) significantly increased (PÂ <Â 0.05) SCF mRNA and SCF protein levels. These findings indicate that AMH differentially regulates the fate of Sertoli cells in vitro by promoting proliferation at low concentrations and apoptosis at high concentrations. In addition, AMH increased the expression of SCF, an important regulator of Sertoli cell development. Therefore, AMH may play a role in Sertoli cell development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 174, November 2017, Pages 133-140
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 174, November 2017, Pages 133-140
نویسندگان
Zia ur Rehman, Tesfaye Worku, John S. Davis, Hira Sajjad Talpur, Dinesh Bhattarai, Ishwari Kadariya, Guohua Hua, Jing Cao, Rahim Dad, Farmanullah Farmanullah, Tarique Hussain, Liguo Yang,